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. 2014 Aug;58(8):4814–4825. doi: 10.1128/AAC.02573-14

TABLE 1.

E. coli and Klebsiella spp. isolated from patients receiving treatments in a UMMC ICU, that carry the FOX beta-lactamase gene

Species Isolatea Sample date (mo/day/yr)b Presence of FOXc Patient age (yr) Patient genderd ICUe Length of hospital stay (days) β-lactam(s) administered during stayf Allele no. for amplicon PCR positive forg:
 No. of plasmids Plasmid Inc type(s)h Multilocus STi
TEM SHV KPC
E. coli 4541-1 02-16-2003 Admission 77 F MICU 4 PIP 12 3 A/C 216
4552-1 02-18-2003 Admission 77 F MICU 4 PIP 12 3 A/C 216
10635 12-24-2004 Admission 78 F MICU 29 IPM 3 A/C, Frep, B/O, L/M 372
9782 09-20-2004 Admission 82 M MICU 9 IPM, PIP 7 3 A/C, Frep, L/M 372
2215 05-14-2002 Admission 74 F MICU 9 PIP 1 A/C 599
3866 12-03-2002 Acquisition 78 M SICU 35 CFZ, IPM, PIP, TIM 1 9 A/C, FIB, Frep 69
9499-3 08-18-2004 Admission 49 F MICU 14 None 1 7 3 A/C, Frep, L/M 372
7996-1 03-13-2004 Admission 54 F SICU 14 IPM 2 A/C 127
11366-2 03-22-2005 Admission 72 M MICU 19 PIP 1 A/C 156
11117 02-20-2005 Admission 77 M MICU 10 PIP 1 5 A/C, FIA, Frep 131
10505 12-11-2004 Admission 76 F SICU 8 PIP 5 A/C, Frep 167
7856 02-28-2004 Admission 71 M MICU 5 IPM 2 A/C, FIA, Frep 131
10376 11-25-2004 Admission 69 F MICU 1 None 4 A/C, FIA, FIB, Frep ND
10810 01-14-2005 Admission 77 F MICU 15 CRO 1 10 A/C, FIA, FIB, Frep 131
K. pneumoniae 4006 12-18-2002 Acquisition 48 M SICU 11 None 11 3 A/C 54
10830 01-19-2005 Acquisition 60 M SICU 29 FEP, IPM, PIP 26 3 A/C 34
8746-1 06-04-2004 Admission 68 F MICU 6 PIP 27 3 A/C 295
1830-2 03-28-2002 Admission 77 M MICU 3 TIM 1 4 A/C 327
4541-2 02-16-2003 Admission 77 F MICU 4 PIP 12 6 A/C 1125
11227-1 03-04-2005 Admission 53 F MICU 10 AMP 26 2 9 A/C, N 228
11646 04-21-2005 Admission 44 M MICU 6 PIP 26 2 9 A/C, N 228
9302 07-30-2004 Admission 60 M MICU 4 PIP 11 2 A/C 429
8657-2 05-25-2004 Admission 75 F MICU 23 PIP 7 A/C, N 1123
7699 02-11-2004 Admission 63 F MICU 9 IPM 11 7 A/C, N 1123
8068 03-23-2004 Admission 56 F MICU 10 IPM 11 7 A/C, N 1123
11225-1 03-05-2005 Admission 81 F MICU 2 None 11 2 A/C 664
11366-3 03-22-2005 Admission 72 M MICU 19 PIP 1 A/C 628
10332 11-20-2004 Admission 63 M MICU 18 IPM, PIP 11 3 A/C 664
Klebsiella sp. 10982 02-02-2005 Admission 57 M SICU 13 CFZ —j 7 A/C 1155
K. oxytoca 11492-1 04-06-2005 Acquisition 64 M MICU 57 PIP, FEP 5 A/C ND
a

Isolates that were analyzed by genome sequencing are indicated in bold. Italics denote isolates obtained from the same patient, and underlining denotes isolates obtained from another patient.

b

The sample date denotes the date that swab sampling was performed on the patient.

c

“Admission” indicates that FOX was detected from a swab sampled shortly after admission to the ICU, while “acquisition” indicates that the FOX gene was not detected initially but was present in subsequent swabs.

d

F, female; M, male.

e

MICU, medical intensive care unit; SICU, surgical intensive care unit.

f

PIP, piperacillin; IPM, imipenem; CFZ, cefazolin; TIM, ticarcillin-clavulanic acid; CRO, ceftriaxone; FEP, cefepime; AMP, ampicillin.

g

The TEM, CTX-M, SHV, KPC, and CARB β-lactamases were detected by PCR as present or absent (cells with no data). Allele numbers are designated for positive PCR amplicons that were sequenced or extracted from the draft genome.

h

The plasmid incompatibility type (Inc type) was determined by PCR detection using genomic DNA of the wild-type isolates.

i

ST is the sequence type (MLST). ND, could not be determined.

j

—, the LEN-26 β-lactamase of 10982 was PCR amplified using the SHV primers.