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ΔprkA mutants are hypersusceptible to β-lactam antibiotics. Cultures of wild-type (wt), Δprk, or complemented ΔprkA L. monocytogenes bacteria grown overnight were back-diluted and treated with 10-fold serial dilutions of ampicillin (A), ceftriaxone (B), cephalexin (C) vancomycin (D), or kanamycin (E). Antibiotic concentrations are in μg/ml. Growth was analyzed for 12 h at 15-min intervals. Data are representative of at least 3 independent repeat experiments.
