FIG 2.

Staurosporine sensitizes L. monocytogenes to β-lactam antibiotics. (A to E) Cultures of wild-type L. monocytogenes grown overnight were back-diluted and treated with 10-fold serial dilutions of ampicillin (A), ceftriaxone (B), cephalexin (C), vancomycin (D), or kanamycin (E) in the presence or absence of 10 μM staurosporine (str). (F) Cultures of S. aureus grown overnight were back-diluted and treated with ceftriaxone in the presence or absence of 10 μM staurosporine. Antibiotic concentrations are in μg/ml. Growth was analyzed for 12 h at 15-min intervals. Data are representative of at least 3 independent repeat experiments.