TABLE 1.
Primers used in this study
| Primer | Sequence (5′–3′)a | Reference |
|---|---|---|
| MLR50 (prkAribo A) | GGCCGGGCCCGGAAATCATAAAAAATTTATTTGC | This study |
| MLR51 (prkAribo B) | CTTAATCGCTTACCAATCATCATCTTGTTGTTACCTCCTTAGCA | This study |
| MLR52 (prkAribo C) | TGCTAAGGAGGTAACAACAAGATGATGATTGGTAAGCGATTAAG | This study |
| MLR53 (prkAribo D) | GGCCCTCGAGTAATTTGGATAAGGGACTGTAC | This study |
| BK38 (prkA KO A) | ATATTATCTAGAGTACCATTGACAAGGAAGAAAATGAAACG | This study |
| BK39 (prkA KO B) | GCACATTTCCTCCGTTCTATTTTTAATTTGGAATCATCATGAAGCATCCCTCCCTTTCTG | This study |
| BK40 (prkA KO C) | CAGAAAGGGAGGGATGCTTCATGATGATTCCAAATTAAAAATAGAACGGAGGAAATGTGC | This study |
| BK41 (prkA KO D) | TATAATTCTAGAACGTCAATATGGATGTAATCTGCACCG | This study |
| JDS50 (PrkA KD F) | ATATTATGGATCCATGATGATTGGTAAGCGATTAAGCGATCGAT | This study |
| JDS54 (PrkA KD R) | ATTATACAATTGTTTCTTTTTCTTGCTCATTTTTTTCTTTTTCTTATCTTTTTTCTC | This study |
| JDS52 (Lmo0618 KD F) | ATATTATGGATCCATGGGAGAAATGACACTTGCTTTTATAGAAGAACA | This study |
| JDS55 (Lmo0618 KD R) | ATTATACAATTGGCCCTCTGTTGGTGGGCTGAAT | This study |
| SA-STPK-F (Stk1 KD F) | TAGGATCCATGATAGGTAAAATAATAAATGAAC | This study |
| SA-STPK-R (Stk1 KD R) | TATAGAATTCTTATCGTGTTGATTTCTTTTTAGGTTTTG | This study |
a
Boldface type indicates restriction sites.