TABLE 3.

FKS sequencing results^a

Anidula MIC (μg/ml)	Caspo MIC (μg/ml)	Mica MIC (μg/ml)	Flucon MIC (μg/ml)	Gene and hot spot region(s)
				FKS1 HS1	FKS2 HS1
0.125	0.25	0.008	128	WT	WT
0.015	0.25	0.008	32	WT	WT
0.03	0.5	0.008	4	WT	WT
0.016	0.25	0.008	4	WT	WT
0.125	0.25	0.016	1	WT	WT
0.016	0.5	0.016	64	WT	WT
0.03	0.25	0.016	8	WT	WT
0.125	0.25	0.016	8	WT	WT
0.03	0.25	0.03	4	WT	WT
0.25	0.125	0.03	128	WT	WT
0.125	0.25	0.03	128	WT	WT
0.125	0.25	0.03	4	WT	WT
0.125	0.25	0.03	4	WT	WT
0.06	0.25	0.06	4	WT	WT
0.125	0.25	0.06	64	WT	WT
0.125	0.5	0.06	64	WT	WT
0.25	0.125	0.06	4	WT	WT
0.25	0.125	0.06	4	F L I L S L R Y P	WT
0.5	0.25	0.125	2	WT	F L I L F L R D P
0.03	0.25	0.125	128	WT	WT
0.5	0.5	0.125	64	F L I L P L R D P	WT
0.5	0.125	0.125	64	F L I L P L R D P	WT
0.125	0.5	0.125	16	WT	WT
0.125	0.125	0.125	8	WT	WT
0.06	0.06	0.125	4	WT	WT
0.5	0.5	0.125	4	WT	WT
2	2	0.25	0.25	WT	F L I L S L R D H
1	0.5	0.25	256	WT	Y L I L S L R D P
1	2	0.25	256	WT	Y L I L S L R D P
0.06	0.125	0.25	64	F L I L S L G D P	WT
2	2	0.25	64	WT	F L I L F L R D P
1	1	0.25	32	WT	S L I L S L R D P
0.06	0.125	0.25	32	F L I L S L G D P	WT
1	1	0.25	16	F L I L S L R V P	WT
1	1	0.25	8	WT	Y L I L S L R D P
0.06	0.125	0.25	8	WT	WT
0.06	0.06	0.25	8	WT	WT
0.03	0.06	0.25	4	I634V	WT
0.03	0.03	0.25	4	WT	WT
1	2	0.25	4	WT	Y L I L S L R D P
0.5	1	0.25	4	WT	Y L I L S L R D P
2	2	0.5	2	WT	F L I L P L R D P
0.5	0.25	0.5	1	F L I L S L G D P	F L I L S L R V P
0.25	0.25	0.5	128	F L I L S L G D P	WT
1	1	0.5	128	WT	delF658
0.03	0.06	0.5	8	WT	WT
0.125	0.125	0.5	4	F L I L P L R D P	F L I L S L R V P
0.03	0.03	0.5	4	WT	WT
1	0.5	1	4	F L I L P L R D P	WT
0.25	0.5	1	2	F L I L S L G D P	WT
2	8	1	256	F L I L P L R D P	WT
2	4	1	64	F L I L P L R D P	F L I L S L R V P
2	16	1	64	WT	F L I L P L R D P
1	4	1	64	WT	F L I L P L R D P
2	2	1	4	WT	WT
2	4	2	4	WT	F L I L P L R D P
0.5	1	2	4	WT	F L I L P L R D P
4	8	2	128	WT	F L I L P L R D P
2	1	2	128	WT	F L I L P L R D P
2	>16	2	128	WT	F L I L P L R D P
2	>16	2	64	WT	F L I L P L R D P
4	>16	2	16	WT	F L I L P L R D P
4	>16	2	16	F L I L P L R D P	WT
4	16	2	8	WT	F L I L P L R D P
4	>16	2	8	WT	delF658
1	16	2	8	WT	F L I L P L R D P
4	>16	2	8	WT	F L I L P L R D P
2	16	2	4	WT	F L I L P L R D P
4	>16	4	4	WT	F L I L P L R D P
2	8	4	4	WT	F L I L P L R D P
0.5	16	4	256	WT	F L I L P L R D P
2	16	4	128	WT	F L I L P L R D P
4	>16	4	64	WT	F L I L P L R D P
2	16	4	32	WT	F L I L P L R D P
4	16	4	32	WT	F L I L P L R D P

^aAmong the 77 isolates with at least one echinocandin MIC in the intermediate or resistant range, FKS sequencing identified 47 with FKS mutations. Three of the isolates had a mutation that was one amino acid outside the recognized hot spot region. All of the mutations were in FKS1 hot spot 1 or FKS2 hot spot 1. This includes 47/55 (85%) isolates that showed resistance to at least one echinocandin. Anidula, anidulafungin; Caspo, caspofungin; Mica, micafungin; Flucon, fluconazole; WT, wild type. Boldface indicates the amino acid that has been changed in the hot spot.