TABLE 2.
Kinetic parameters of purified β-lactamases OXA-24 and OXA-207a
| Antimicrobial agent | OXA-24b |
OXA-207 |
||||
|---|---|---|---|---|---|---|
| kcat (s−1)c | Km (μM) | kcat/Km (mM−1 s−1) | kcat (s−1) | Km (μM) | kcat/Km (mM−1 s−1) | |
| Ampicillin | 223 (92.9) | 80 (15) | 2,75 | 337 (6.7) | 151 (15.4) | 2,232 |
| Oxacillin | 257 (0.086) | 1,272 (222) | 202 | 383 (28.5) | 43 (8.3) | 8,907 |
| Imipenem | 1.62 (0.47) | 0.58 (0.084) | 2,786 | 0.41 (0.01) | 0.37 (0.07) | 1,108 |
| Meropenem | 0.37 (0.1) | 0.0099 (0.0014) | 37,869 | 0.043 (0.002) | 0.008 (0.001) | 5,375 |
a
Data are the means (standard deviations shown in parentheses) of three independent experiments.
b
Data for OXA-24 were obtained from reference 21.
c
Estimated kcat value.