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Time-kill assays with DAP (5× the MIC) with or without AMP (64 μg/ml). Bacteria were grown in Mueller-Hinton broth (MHB) supplemented with calcium (50 mg/liter). The E. faecium strains are indicated at the top of each panel. AMP, ampicillin; DAP, daptomycin. The limit of detection was 200 CFU/ml.
