TABLE 1.
Shifts in phosphate atom positions induced by Strr mutationsa
| Residue | Distance (Å) |
|||||
|---|---|---|---|---|---|---|
| U13C (4DUY) | U20G (4DV0) | C912A (4DV2) | A914G (4DV4) | A915G (4DV6) | ΔrsmG (4NXM) | |
| U13 | 0.5 | 0.8 | 0.2 | 0.5 | 0.6 | 0.2 |
| U14 | 0.9 | 0.6 | 0.3 | 0.2 | 1.0 | 0.0 |
| G15 | 0.5 | 0.6 | 0.3 | 0.1 | 0.5 | 0.1 |
| C526 | 0.3 | 0.6 | 0.4 | 0.2 | 0.2 | 0.1 |
| m7G527 | 1.1 | 0.3 | 0.2 | 0.7 | 0.8 | 0.2 |
| C912 | 0.6 | 0.5 | 1.4 | 0.3 | 0.8 | 0.2 |
| A913 | 1.1 | 1.1 | 0.3 | 0.4 | 1.2 | 0.1 |
| A914 | 0.7 | 0.4 | 0.5 | 1.0 | 0.5 | 0.4 |
| A915 | 0.8 | 1.0 | 0.2 | 0.5 | 0.8 | 0.2 |
| C1490 | 1.2 | 0.4 | 1.4 | 0.4 | 1.1 | 0.2 |
| G1491 | 1.3 | 0.6 | 3.4 | 0.6 | 1.4 | 0.4 |
| A1492 | 1.9 | 0.7 | 3.6 | 0.7 | 2.3 | 0.7 |
| A1493 | 0.8 | 1.8 | 2.9 | 0.9 | 1.5 | 0.4 |
a
Coordinate errors are provided in Table S1 in the supplemental material. Root mean square deviation values for the overall alignments were 0.219 (U13C), 0.262 (U20G), 0.247 (C912A), 0.204 (A914G), 0.250 (A915G), and 0.188 (ΔrsmG). Residues involved in streptomycin binding are U14, C526, m7G527, A914, C1490, and G1491. PDB accession numbers are given in parentheses.