TABLE 3.
Streptomycin binding site dimensions in ribosome-streptomycin complexesa
| Residue pair | Distance (Å) |
||||||
|---|---|---|---|---|---|---|---|
| Wild type (4DR3) | U13C (4DUZ) | U20G (4DV1) | C912A (4DV3) | A914G (4DV5) | A915G (4DV7) | ΔrsmG (4NXN) | |
| U14-C526 | 12.1 | 12.4 | 12.3 | 12.2 | 12.4 | 12.2 | 12.2 |
| U14-m7G527 | 8.8 | 9.0 | 9.0 | 8.9 | 9.0 | 8.8 | 8.6 |
| U14-A914 | 6.9 | 7.4 | 7.2 | 6.9 | 7.0 | 7.3 | 7.0 |
| U14-C1490 | 18.5 | 19.0 | 18.7 | 18.3 | 18.4 | 18.4 | 18.3 |
| U14-G1491 | 16.9 | 17.5 | 16.9 | 16.8 | 16.5 | 16.9 | 16.8 |
| C526-A914 | 6.1 | 5.9 | 6.1 | 6.1 | 6.1 | 5.9 | 6.2 |
| C526-C1490 | 17.0 | 16.7 | 17.0 | 17.2 | 17.1 | 17.0 | 16.9 |
| C526-G1491 | 14.0 | 14.0 | 13.9 | 14.0 | 14.0 | 14.2 | 13.8 |
| m7G527-A914 | 6.7 | 6.8 | 6.9 | 6.7 | 6.7 | 6.7 | 6.9 |
| m7G527-C1490 | 18.8 | 18.6 | 19.2 | 19.0 | 19.0 | 18.8 | 18.8 |
| m7G527-G1491 | 15.1 | 15.0 | 15.3 | 15.1 | 15.1 | 15.1 | 14.9 |
| A914-C1490 | 15.4 | 15.4 | 15.5 | 15.6 | 15.6 | 15.5 | 15.4 |
| A914-G1491 | 13.8 | 14.0 | 13.8 | 13.8 | 13.6 | 14.0 | 13.9 |
a
Distances between phosphate atoms are indicated for each pair of residues in the streptomycin binding site in 30S subunits complexed with streptomycin. Coordinate errors are provided in Table S1 in the supplemental material. PDB accession numbers are given in parentheses.