FIG 6.

Effect of UPC2A disruption on both baseline and sterol biosynthesis inhibitor-induced expression of ergosterol biosynthesis genes. RNA was isolated from mid-log-phase cultures in parent clinical isolates, Δupc2A mutants, and revertants. qRT-PCR analysis of relative gene expression was performed. (A) SM1, Δupc2A mutant, and revertant gene expression. Gene expression was normalized to that in the untreated SM1 sample for each ergosterol biosynthesis gene. (B) SM3, Δupc2A mutant, and revertant gene expression. Gene expression was normalized to that in the untreated SM3 sample for each ergosterol biosynthesis gene.