FIG 2.

Activity of fexinidazole metabolites against intracellular amastigotes of Trypanosoma cruzi. Peritoneal murine macrophages were infected with T. cruzi Y strain and incubated with different concentrations of fexinidazole metabolites or benznidazole for 72 h. Methanol-fixed cells were stained with Giemsa. Arrows, intracellular parasites. (A, C, and E) Drug activity at the 90% inhibitory concentration (IC₉₀) (note the absence of parasites); (B, D, and F) drug activity at the IC₅₀; (G) uninfected cells; (H) infected and untreated cells. No cytotoxic effects were observed in host cells.