Dose-dependent effects of d-AAs against S. aureus and P. aeruginosa biofilms. Screening of dispersive activity of individual d-amino acids, d-methionine (d-Met), d-phenylalanine (d-Phe), and d-tryptophan (d-Trp) (at concentrations ranging from 0.01 to 50 mM) against biofilms of two clinical methicillin-resistant S. aureus (MRSA) isolates (top panels) and two multidrug-resistant (MDR) isolates of P. aeruginosa (bottom panels). Biofilm dispersal was assessed by measuring the absorbance of solubilized crystal violet from stained biofilms following overnight treatment with d-AAs at 570 nm. *, P < 0.05 versus untreated control. CV, crystal violet.