FIG 1.

Radicicol-induced death of Plasmodium falciparum. (A) Structure of radicicol showing its functional groups. (B) Inhibition of P. falciparum 3D7 by radicicol. Synchronized schizont stage-specific parasites were incubated with increasing doses of radicicol for 48 h. Parasitemia levels were determined by microscopy using Giemsa stain, and percent inhibition was calculated. Mean and standard errors from two experiments were plotted. (C) Parasitemia was determined using SYBR green I-based fluorescence measurement (repeated two times) as described in Materials and Methods. (D) Linear relationship between parasitemia and fluorescence values as used in this study.