FIG 4.

Characterization of radicicol-treated parasites. (A) Morphology of parasites treated with 1.5 μM radicicol showed no change compared to the untreated parasites. (B) The ploidy of the treated parasites is comparable to that of the untreated parasites. A minimum of 100 parasitic schizont nuclei in each case (untreated and radicicol treated) were counted. (C) Synchronized schizont-stage parasites were treated with 1.5 μM radicicol for 40 h and genomic DNA samples from treated and untreated cultures were subjected to Southern hybridization using PfOrc1 (nuclear probe), PfCox3 (mitochondrial probe), or PfSufB (apicoplast probe). The experiment was repeated two times. One of the Southern blots is shown. (D) Synchronized schizont-stage parasites were treated with 170 nM 17AAG for 48 h and genomic DNA from treated and untreated cultures was subjected to Southern hybridization in a similar fashion using the above-mentioned nuclear, mitochondrial, and apicoplast probes.