FIG 2.
Knockdown of SAMHD1 has differential effects on NRTI activation. PMA-differentiated parental and THP-1KD-SAMHD1 cells were treated with 1.5 μM (0.5 μCi) [2-14C]AZT or [4-14C]d4T. After 24 h at 37°C, the cells were lysed, the dNTP and NRTI metabolites were separated by anion-exchange HPLC (DNAPac PA100 column), and the NRTI metabolites in the collected fractions were quantified with a liquid scintillation counter. Data represent the mean ± standard deviation (SD) from 2 independent experiments. *, P < 0.05.