TABLE 4.

Apparent average melting temperatures of VIM-7 and mutants as found by ThermoFluor assays^a

Protein	Tm (°C) for:		ΔTm (°C)
	pH 7.5	pH 7.5 + ZnCl2
tVIM-7	55.3 ± 0.2	48.4 ± 0.2	−6.9
tVIM-7-D120A	48.5 ± 0.1	46.1 ± 0.1	−2.4
tVIM-7-H224Y	62.9 ± 0.1	60.2 ± 0.1	−2.7
tVIM-7-F218Y	57.1 ± 0.8	57.0 ± 0.6	−0.1
VIM-7	57.4 ± 0.2	53.8 ± 0.2	−3.6

^aThe assays were performed in 50 mM HEPES (pH 7.5) with 400 μM ZnCl₂ or 1 mM EDTA (data not shown). The experiments were performed in duplicate.