FIG 3.

Effects of LL-37, P60.4Ac, and P10 on biofilms and biofilm-associated bacteria. (A) Breakdown of existing biofilms. Biofilms of LUH14616 were grown for 24 h and subsequently incubated for 4 h with peptides (concentrations, 1.5, 3, 6, 12, 25, 50, and 100 μM). Next, bacteria were scraped from the wells, and the number of viable bacteria was assessed microbiologically. The data are expressed as the percentage of surviving bacteria compared to the control biofilms. (B and C) Effects of peptides on biofilm-associated bacteria from 24-h-matured biofilms. The bacteria were scraped from the wells, diluted to 1 × 10⁶ CFU/ml, and incubated for 1 h (B) and 4 h (C) with peptides (0.2, 0.4, 0.8, 1.6, 3.2, and 32 μM). White bars, LL-37; gray bars, P60.4Ac; black bars, P10; striped bar, control (no peptide present). The results are displayed as medians and interquartile ranges of 4 to 5 independent experiments. * indicates CFU/ml significantly less than that in the absence of peptide.