FIG 7.

Colony morphology and biofilm formation of V. salmonicida wild-type LFI1238 and the different mutants. (A) The colony morphology of the different strains shows that the disruption of sypC, sypP, and sypQ in the ΔlitR mutant alleviates the rugose appearance. (B) Quantitation of the biofilms formed in L15-M and SWT medium after 3 days of static incubation. The error bars show the standard deviations of three biological replicates. (C) Phase-contrast microscopy (Leica) shows that disruption of syp results in a flat and regular biofilm when formed in SWT medium. The white arrows point to irregular mushroom-like structures in the biofilm. All experiments were performed at 4°C.