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Verification of the constructed S. meliloti lsrB deletion mutant (lsrB1-2 mutant). (A) Genomic organization of the wild type (Wt; S. meliloti 1021) and the lsrB1-2 mutant. (B and C) The lsrB1-2 mutant was confirmed using PCR (B) and RT-PCR (C). The numbers 1 to 6 indicate primers used for PCR.
