TABLE 2.
Primersa
| Name | Sequence | Purpose |
|---|---|---|
| P1 | 5′-GGAATTCCGACCTCGTAACCGATGTCGA-3′ | Construction of the lsrB deletion mutant |
| P2 | 5′-AAAGGCTCAGCCGGAGAAGC-3′ | Construction of the lsrB deletion mutant |
| P3 | 5′-CTCCGGCTGAGCCTTTCGATCCGTTCAATTGCCCG-3′ | Construction of the lsrB deletion mutant |
| P4 | 5′-GCTCTAGAGCACGGCGAGATGAGATGAAA-3′ | Construction of the lsrB deletion mutant |
| P5 | 5′-GGAATTCCATATGGGGGATTCTATGTCGCTGGACT-3′ | Cloning of lsrB gene |
| P6 | 5′-GCTCTAGATCAGAAGTTCCAGTTTCTCGCTTTA-3′ | Cloning of lsrB gene |
| P7 | 5′-TGCACTGCAGCCGGCGACGTGACCGAC-3′ | Cloning of lsrB promoter |
| P8 | 5′-CGGGATCCGGGCGTGCCGATGAAAGA-3′ | Cloning of lsrB promoter |
| P9 | 5′-TGCACTGCAGTTGCCCAAATCCCCAATCCT-3′ | Cloning of trxB promoter |
| P10 | 5′-CGGGATCCGCTTTTGTCCTTCGTCCGCC-3′ | Cloning of trxB promoter |
| P11 | 5′-CGGGATCCTTGCCCAAATCCCCAATCCT-3′ | Cloning of lrp3 promoter |
| P12 | 5′-TGCACTGCAGGCTTTTGTCCTTCGTCCGCC-3′ | Cloning of lrp3 promoter |
| P13 | 5′-TGCACTGCAGATTTTGTTATTGAAGAACTGACCG-3′ | Cloning of lpsC promoter |
| P14 | 5′-CGGGATCCGAGACTCTCGATGCACTTGCC-3′ | Cloning of lpsC promoter |
| P19 | 5′-TCCGGAAATCCAGGTACAGC-3′ | qRT-PCR of lsrB |
| P20 | 5′-ATGCGATATGCGCGGAT-3′ | qRT-PCR of lsrB |
| P21 | 5′-TCAGAAAGGGACCGACCTCTT-3′ | qRT-PCR of lpsB |
| P22 | 5′-CCAGTCGGGAATATTCGTGTG-3′ | qRT-PCR of lpsB |
| P23 | 5′-GCCTATCTCGGCAAGTGCAT-3′ | qRT-PCR of lpsC |
| P24 | 5′-GATGTTGAAGCACCAGGGCT-3′ | qRT-PCR of lpsC |
| P25 | 5′-ATCTTTGGCTGCTTGGCGA-3′ | qRT-PCR of lpsE |
| P26 | 5′-ATGAACCATTGCGGCCCTT-3′ | qRT-PCR of lpsE |
| P27 | 5′-ATCGTGCGCTTCTGAATGC-3′ | qRT-PCR of lrp3 |
| P28 | 5′-TCCTGAAAGCTCGCAAGGTT-3′ | qRT-PCR of lrp3 |
| P29 | 5′-AAGGTGATCTGGGACCACGA-3′ | qRT-PCR of trxB |
| P30 | 5′-GCGAAGTTTGCCCTTGAAGA-3′ | qRT-PCR of trxB |
| P31 | 5′-GATCGTCATGTAGCGCAGGA-3′ | qRT-PCR of rpsF |
| P32 | 5′-CCTCGCTCGGCAGGACAT-3′ | qRT-PCR of rpsF |
| P33 | 5′-CATGGCTACATGCTGACAGCCTA-3′ | 5′-RACE of outRAP |
| P34 | 5′-CGCGGATCCACAGCCTACTGATGATCAGTCGATGGAAA-3′ | 5′-RACE of inRAP |
| P35 | 5′-TTGTCGAGGATGAGCTGTAC-3′ | 5′-RACE of lsrB |
| P36 | 5′-TTGGCTTGTCCGTCGTCT-3′ | 5′-RACE of lsrB |
| P37 | 5′-AAGGTCGGCTCGGTTTCGAT-3′ | 5′-RACE of trxB |
| P38 | 5′-GGTTACGAGGTCGTTGAC-3′ | 5′-RACE of trxB |
| P39 | 5′-AAAGTCCGATTCCCCGGA-3′ | 5′-RACE of lrp3 |
| P40 | 5′-TTGAGATTGGCGTCGGACTG-3′ | 5′-RACE of lrp3 |
| P41 | 5′-CGATCTCGTCTGATGCTGC-3′ | 5′-RACE of lpsC |
| P42 | 5′-TACATGAAACGGATGGGCCA-3′ | 5′-RACE of lpsC |
| P43 | 5′-TGGGACAAACTGCGCATTT-3′ | RT-PCR of lsrB |
| P44 | 5′-GCTTTCGAGCTTCATCAGCAC-3′ | RT-PCR of lsrB |
| P45 | 5′-ACATCCGACATGGCAATCG-3′ | RT-PCR of trxB |
| P46 | 5′-AGGTATTTCTCCGCTTCGAGC-3′ | RT-PCR of trxB |
| P47 | 5′-ATCGTGCGCTTCTGAATGC-3′ | RT-PCR of lrp3 |
| P48 | 5′-TCCTGAAAGCTCGCAAGGTT-3′ | RT-PCR of lrp3 |
| P49 | 5′-GCCTATCTCGGCAAGTGCAT-3′ | RT-PCR of lpsC |
| P50 | 5′-GATGTTGAAGCACCAGGGCT-3′ | RT-PCR of lpsC |
| P51 | 5′-CATATTCACTTCGGCACGGAG-3′ | RT-PCR of lpsD |
| P52 | 5′-GCAACTGCCAGGCCATTATG-3′ | RT-PCR of lpsD |
| P53 | 5′-CAAGGTTTTTGTGCATGCAACTGC-3′ | EMSA |
| P54 | 5′-GTTCCAAAAACACGTACGTTGACG-3′ | EMSA |
a
Italics represent restriction endonuclease sites.