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. 2014 Sep;80(17):5503–5514. doi: 10.1128/AEM.01177-14

TABLE 1.

Primers used for PCR and sequencing of the 11 loci

Locus Step Primer sequence (5′–3′)
Forward Reverse
atpA PCR ATTGGWGAYCGTCAAACWGG CCAAAYTTAGCRAAHGCTTC
dnaK PCR GGWACYACNAAYTCDTGTGT TCWATCTTMGCTTTYTCAGC
glyA PCR CAYTTAACWCAYGGWTCDCC ACCATRTTTTTRTTTACHGT
gyrB PCR AGTATYCARGCRCTRGAAGG GTWCCTCCTTCRTGYGTRTT
ileS PCR CCWACHTTTGGWGCHGAYGA GAATCRAACCAWACATCAAT
infB PCR ATGCCDCAAACWAAAGARGC GTAATHGCTCCAACYCCTTT
rlmN PCR GCKTGTGTDTCDAGYCARGT CCRCADGCDGCATCWATRTC
tgt PCR GAAACWCCWATWTTYATGCC TAYAWYTCTTCNGCWGGTTC
trpB PCR GTWGCNCGWATGAAAATGYT CCWGGRTARTCYAATCCTGC
tuf PCR AGAGAWTTATTRTCTTTCTA GTTACCTGACCWGCWCCWAC
yqfO PCR GCBGAARRTTTTGAYAAYGT AYTTCRTARGCDACYTCTTC
All Sequencing CAGGAAACAGCTATGACC TGTAAAACGACGGCCAGT