TABLE 1.
Locus | Step | Primer sequence (5′–3′) |
|
---|---|---|---|
Forward | Reverse | ||
atpA | PCR | ATTGGWGAYCGTCAAACWGG | CCAAAYTTAGCRAAHGCTTC |
dnaK | PCR | GGWACYACNAAYTCDTGTGT | TCWATCTTMGCTTTYTCAGC |
glyA | PCR | CAYTTAACWCAYGGWTCDCC | ACCATRTTTTTRTTTACHGT |
gyrB | PCR | AGTATYCARGCRCTRGAAGG | GTWCCTCCTTCRTGYGTRTT |
ileS | PCR | CCWACHTTTGGWGCHGAYGA | GAATCRAACCAWACATCAAT |
infB | PCR | ATGCCDCAAACWAAAGARGC | GTAATHGCTCCAACYCCTTT |
rlmN | PCR | GCKTGTGTDTCDAGYCARGT | CCRCADGCDGCATCWATRTC |
tgt | PCR | GAAACWCCWATWTTYATGCC | TAYAWYTCTTCNGCWGGTTC |
trpB | PCR | GTWGCNCGWATGAAAATGYT | CCWGGRTARTCYAATCCTGC |
tuf | PCR | AGAGAWTTATTRTCTTTCTA | GTTACCTGACCWGCWCCWAC |
yqfO | PCR | GCBGAARRTTTTGAYAAYGT | AYTTCRTARGCDACYTCTTC |
All | Sequencing | CAGGAAACAGCTATGACC | TGTAAAACGACGGCCAGT |