FIG 4.
Determination of the cell wall lytic activity of TgaA protein. A recombinant form of TgaA (SPPelB-TgaA) was expressed in E. coli XL1 through vector pAK-tga (A), resulting in altered cell morphology after 24 h of growth at 37°C in 2× YT medium (B). After wild-type XL1 cells were incubated at 37°C for 4 h in the presence of 50 μg of purified SPPelB-TgaA, the addition of 10% SDS to a final concentration of 0.2% determined instantaneous complete cell lysis (C). Vector pAK-tga was obtained by cloning gene tgaA in the empty vector pAK400.