FIG 5.

Double expression reporter system for the determination of the peptidoglycan lytic activity of TgaA. A dose-dependent increase of bioluminescence by IPTG induction in E. coli SNO301 containing pAK-tga and pBlaLux1 was observed. The same strain containing only vector pBlaLux1 was used as a control. The addition of IPTG to E. coli SNO301 containing only pBlaLux1 (and not pAK-tga) determined a reduction of the light emission (dotted lines). RLU, relative luminescence units. Each point in the plot represents the mean of the values obtained in one representative experiment conducted in quadruplicates. The variation between the replicates was less than 10%.