TABLE 2.

Primer sets used in this study

Target	Amplicon size (bp)	Oligonucleotide	Sequence (5′ to 3′)a	Tab (°C)	Reference(s)
sul1	158	sul1-FW	CACCGGAAACATCGCTGCA	65	32
		sul1-RV	AAGTTCCGCCGCAAGGCT	65	32
sul2	190	sul2-FW	CTCCGATGGAGGCCGGTAT	65	32
		sul2-RV	GGGAATGCCATCTGCCTTGA	65	32
qnrA	543	qnrA-F	GATAAAGTTTTTCAGCAAGAGG	56	33
		qnrA-R	ATCCAGATCGGCAAAGGTTA	56	33
qnrB	497	qnrB-F	AGCGGCACTGAATTTAT	56	33
		qnrB-R	GTTTGCTGCTCGCCAGTC	56	33
qnrS	600	qnrS-F	GGAAACCTACAATCATACATA	56	33
		qnrS-R	GTCAGGATAAACAACAATACC	56	33
Bacterial 16S rRNA gene	1,465	27F	GAGTTTGATCMTGGCTCAG	58	34
		1492R	GGYTACCTTGTTACGACTT	58	34
	1,324	63fw	CAGGCCTAACACATGCAAGTC	56	35
		1387rev	GGGCGGWGTGTACAAGGC	56	35
	414	101Fc	CCTATCCCCTGTGTGCCTTGGCAGTCTCAGAGTGGCGGACGGGTGAGTAAd	69	36, 37
		515Rc	CCATCTCATCCCTGCGTGTCTCCGACTCAG-MID-CCGCGGCTGCTGGCACe	69	36, 37

^aY = C or T.

^bAnnealing temperature.

^cPrimers for pyrosequencing.

^dRoche 454 pyrosequencing adaptor B is underlined.

^eRoche 454 pyrosequencing adaptor A is underlined. MID, sample-specific extended multiplex identifier (10 nucleotides).