In vivo flux distribution of P. putida KT2440 during growth on glucose (A), fructose (B), and benzoate (C). Molar net fluxes (mmol gCDW−1 h−1) are obtained from the best fit with substrate utilization rates on the respective substrates with a growth rate of 0.1 h−1. The arrows are drawn in proportion to the flux. Metabolites in a gray-shaded background represent the biomass precursor molecules. Broken lines indicate zero flux or nonconsidered reactions. Abbreviations: T3P, triose-3-phosphate; P5P, pentose-5-phosphates; PGA, 3-phosphoglycerate; PO, phosphate/oxygen ratio.