TABLE 1.

Specific activities of haloalkane dehalogenase in P. putida MC4 with various expression constructs^a

Plasmid	DhaA variantb	Promoter	Haloalkane dehalogenase activity (U/mg)
pIT27	DhaA27	trc	0.10 ± 0.02
pIT31	DhaA31	trc	0.16 ± 0.02
pIS27A	DhaA27	dhlA, A variant	0.07 ± 0.01
pIS27B	DhaA27	dhlA, B variant	0.09 ± 0.01
pIS31A	DhaA31	dhlA, A variant	0.06 ± 0.01
pIS31B	DhaA31	dhlA, B variant	0.11 ± 0.02

^aSpecific activities (U/mg protein) were measured with TCP using extracts from cells grown in LB medium.

^bDehalogenase variants described by Pavlova et al. (24).