TABLE 3.
Specific dehalogenase activities in cell extracts of MC4 and its derivativesa
| Strain | Activity (U/mg)b |
|||
|---|---|---|---|---|
| DhaA31 |
DppA | CPA dehalogenase | ||
| With TCP | With DBE | |||
| MC4 | <0.01 | <0.005 | 0.22 ± 0.01 | 1.05 ± 0.09 |
| MC4(pIS31B) | 0.24 ± 0.06 | 1.3 ± 0.3 | 0.14 ± 0.01 | 0.64 ± 0.06 |
| MC4-5222 | 0.02 ± 0.01 | 0.14 ± 0.01 | 0.35 ± 0.05 | 1.27 ± 0.18 |
a
Cells were grown in a medium (MMY) with 4 mM DCP as the carbon source.
b
The activity of DhaA31 was measured with DBE or TCP as the substrate. The activity of DppA was measured with 2,3-dichloropropanol and that of CPA dehalogenase with (S)-2-chloropropionic acid.