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FTIR absorbance spectrum of the second product fraction after incubation of latex milk with active Lcp1VH2 in comparison to the spectrum of latex of the negative control. From a latex emulsion containing 20% (vol/vol) latex, 50 μl/ml was mixed with 15 μg/ml of purified protein (negative control: heat-inactivated enzyme) in Bis-Tris buffer (200 mM, pH 7.0) and incubated for 20 h at 30°C on a rotary shaker (150 rpm). R′ to R′′′, alkyl residues.
