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Temperature (A) and pH (B) optima of purified Lcp1VH2. A 20-μl/ml latex suspension was incubated with 5 μg/ml of purified Lcp1VH2, and oxygen consumption was measured. For determination of the temperature optimum, Bis-Tris buffer (200 mM, pH 7.0) was chosen. For evaluation of the pH optimum of the enzyme, Bis-Tris (▲), PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] (●), and Tris (◼) (each 200 mM) were applied at the corresponding pH values at 23°C.
