TABLE 1.
Strain collection of 83 clinical mold isolates analyzed by MALDI-TOF MS
| Genus | No. of isolates | No. of isolates identified by MALDI-TOF MSa |
No. of misidentificationsb | No. of reference spectrac | |||
|---|---|---|---|---|---|---|---|
| Genus level | Species level (cutoff, 2.0) | Species level (cutoff, 1.7) | No identification | ||||
| Acremonium | 3 | 1 | 0 | 1 | 2 | 0 | 1 |
| Aspergillus | 24 | 24 | 17 | 21 | 0 | 0 | 90 |
| Beauveria | 3 | 1 | 0 | 1 | 2 | 0 | 1 |
| Chrysosporium | 3 | 3 | 2 | 3 | 0 | 0 | 3 |
| Fusarium | 6 | 6 | 6 | 6 | 0 | 0 | 36 |
| Lichtheimia | 3 | 3 | 3 | 3 | 0 | 0 | 11 |
| Mucor | 3 | 1 | 0 | 0 | 2 | 0 | 5 |
| Paecilomyces | 9 | 6 | 6 | 6 | 3 | 0 | 11 |
| Penicillium | 5 | 4 | 2 | 4 | 1 | 1 | 67 |
| Rhizopus | 3 | 3 | 1 | 3 | 0 | 0 | 15 |
| Scedosporium | 6 | 6 | 6 | 6 | 0 | 0 | 6 |
| Schizophyllum | 3 | 3 | 1 | 3 | 0 | 0 | 4 |
| Scopulariopsis | 6 | 0 | 0 | 0 | 6 | 0 | 9 |
| Trichoderma | 3 | 2 | 1 | 2 | 1 | 1 | 3 |
| Trichurus | 3 | 2 | 0 | 0 | 1 | 0 | 1 |
| Total (%) | 83 (100) | 65 (78.3) | 45 (54.2) | 59 (71.1) | 18 (21.7) | 2 (2.4) | 263 |
a
MALDI-TOF MS identification applying a genus cutoff of 1.7 and species cutoffs of 2.0 and 1.7, respectively, using the Filamentous Fungi Library 1.0 (Bruker Daltonik).
b
Misidentifications by MALDI-TOF MS: Penicillium olsonii (assignment by ITS sequence analysis) misidentified as Penicillium brevicompactum and Trichoderma citrinoviride (assignment by ITS sequence analysis) misidentified as Trichoderma koningii.
c
Number of reference spectra in the Filamentous Fungi Library 1.0 (Bruker Daltonik).