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Analysis of CMV standard materials by ddPCR for CMV UL55. (A) WHO dilution series, run in singlet; (B) NIST CMV DNA standard dilution series. Eight replicate PCRs were performed per dilution. (C) Acrometrix CMVtc dilution panel. Eight replicate PCRs were performed per dilution. (D) CMV patient plasma sample dilution series. Each dilution was assayed in 4 replicate reactions for ddPCR and duplicate reactions for real-time PCR.
