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Comparison of within-run (intraassay) variability and between-run (interassay) variability for ddPCR and qPCRa
| Dilution (copies/ml) | CV (%) |
|||
|---|---|---|---|---|
| Interassay |
Intraassay |
|||
| ddPCR | qPCR | ddPCR | qPCR | |
| 150 | 24.1 | 44.0 | 33.5 | 37.7 |
| 300 | 19.8 | 26.4 | 19.0 | 28.1 |
| 1,500 | 8.6 | 13.2 | 11.7 | 14.9 |
Utilizing 3 dilutions of CMV patient plasma sample, each run in triplicate and repeated in four separate runs. CV, coefficient of variation.
