FIG 5.

PI-6 contributes to NRG857c colonization of the ileum and colon. Groups of CD-1 mice were colonized with either wild-type NRG857c or the ΔPI-6 mutant, and fecal shedding was measured. At days 7 and 30, the mice were sacrificed, ileal or colonic contents were removed, and the tissue-associated AIEC burden was determined. (A) Fecal output from days 2 to 14 in mice colonized with strain NRG857 or NRG857ΔPI-6. (B) Tissue-associated AIEC on day 7 (n = 5) and day 30 (n = 10). Data are the means plus standard errors from two separate experiments. Values that are significantly different are indicated by bars and asterisks as follows: *, P < 0.05; **, P < 0.01.