Percentage of protein expressed during coculture or monoculture using various strains of P. gingivalis and F. alocis. HeLa cells were infected with F. alocis ATCC 35896 and D-62D strains (MOI of 1:100 [105 epithelial cells]) in monoculture or coculture with P. gingivalis W83 as previously reported (6). Tandem isobaric mass tagging analysis of cocultures and monocultures was carried out using Orbitrap. (*, P < 0.01.) Bar A, P. gingivalis (33277) plus F. alocis (D-62D) versus P. gingivalis (33277) plus F. alocis (ATCC); bar B, P. gingivalis (33277) plus F. alocis (D-62D) versus P. gingivalis (W83) plus F. alocis (D-62D); bar C, P. gingivalis (33277) plus F. alocis (D-62D) versus F. alocis (D-62D); bar D, P. gingivalis (33277) plus F. alocis (ATCC) versus P. gingivalis (W83) plus F. alocis (ATCC); bar E, P. gingivalis (W83) plus F. alocis (D-62D) versus P. gingivalis (W83) plus F. alocis (ATCC); bar F, P. gingivalis (W83) plus F. alocis (D-62D) versus F. alocis (D-62D); bar G, F. alocis (D-62D).