TABLE 1.
H. pylori cag mutant strains used in the current study
| Description of mutant | Gene mutated | Nucleotides deleted | Insertion position (nucleotide) | Complemented gene | Antibiotic resistance cassettea | Antibiotic resistanceb | Genotype of parental strain |
|---|---|---|---|---|---|---|---|
| Marked mutants | |||||||
| cagC::cat-rdx | cagC | 219 | cat-rdx | Chl | ΔrdxA | ||
| ΔcagM marked | cagM | 70–909 | cat-rdx | Chl | ΔrdxA | ||
| cagT::cat-rdx | cagT | 542 | cat-rdx | Chl | ΔrdxA | ||
| ΔcagV marked | cagV | 139–720 | cat-rpsL | Chl | ΔrpsL | ||
| ΔcagX marked | cagX | 139–1,470 | cat-rpsL | Chl | ΔrpsL | ||
| ΔcagY marked | cagY | 1–5,745 | cat-rpsL | Chl | ΔrpsL | ||
| Δcag3 marked | cag3 | 1–1,203 | cat-rpsL | Chl | ΔrpsL | ||
| Δcag PAI | cag PAI | All | cat-rdx | Chl | ΔrdxA | ||
| Unmarked mutants | |||||||
| ΔcagC unmarked | cagC | 78–341 | Met | ΔrdxA | |||
| ΔcagM unmarked | cagM | 70–909 | Met | ΔrdxA | |||
| ΔcagV unmarked | cagV | 139–720 | Strep | ΔrpsL | |||
| ΔcagX unmarked | cagX | 139–1,470 | Strep | ΔrpsL | |||
| Δcag3 unmarked | cag3 | 1–1,203 | Strep | ΔrpsL | |||
| Complemented mutants | |||||||
| ΔcagC unmarked complemented | cagC | cat | Chl, Met | ΔrdxA | |||
| ΔcagM unmarked complemented | cagM | cat | Chl, Met | ΔrdxA | |||
| cagT::cat-rdx complemented | cagT | cat | Kan, Chl | ΔrdxA | |||
| ΔcagV unmarked complemented | cagV | cat | Chl, Strep | ΔrpsL | |||
| ΔcagX unmarked complemented | cagX | cat | Chl, Strep | ΔrpsL | |||
| Δcag3 unmarked complemented | cag3 | cat | Chl, Strep | ΔrpsL |
a
Mutants generated using the cat-rdx cassette were derived from the ΔrdxA parental strain. Mutants generated using the cat-rpsL cassette were derived from the ΔrpsL parental strain.
b
Chl, chloramphenicol; Met, metronidazole; Strep, streptomycin; Kan, kanamycin.