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. 2014 Aug;82(8):3341–3349. doi: 10.1128/IAI.01795-14

FIG 1.

FIG 1

Urogenital infection course in female mice inoculated intravaginally with the plasmid(−), plasmid(+), and plasmid CDS5 deletion strains of C. trachomatis serovar L2. The graphs on the left depict the mean infectious burden over time in mice inoculated with L2P− (open circles), L2P−.pGFP::SW2 (filled squares), and the derivative of L2P−.pGFP::SW2 but with a deletion mutation in CDS5 (pgp3), L2P−.pCDS5KO (open squares). The graphs on the right depict the percentage of mice that were culture positive at the same time points. Results are for doses of 103, 104, 105, and 106 IFU per mouse (top to bottom; n = 10 mice inoculated per group). There were significant differences between the infectious burden (IFU) over time at the 105 dose in the L2P−. pGFP::SW2 group and that in the L2P− or the L2P−.pCDS5KO group (P < 0.001 and P = 0.004 by two-way ANOVA), but there was no difference between groups receiving L2P− and L2P−.pCDS5KO (P = 0.663). Similarly, there were significant differences between the infection courses in the 106 dose group receiving L2P−. pGFP::SW2 and the group receiving L2P− or L2P−.pCDS5KO (P < 0.001 for both groups), and there was no difference between the L2P− and L2P−.pCDS5KO groups at this dose (P = 0.956). Statistical analyses were not conducted on the 103 and 104 IFU doses due to low numbers of mice sustaining infection, as assessed either by culture or by seroconversion, in the groups inoculated with L2P− and L2P−.pCDS5KO.