FIG 1.

Surface chitin exposure during germination is increased in isolate Af5517 in comparison to that in Af293. (A) Conidia were left dormant or were germinated at 37°C for 5 or 24 h and then analyzed by flow cytometry for changes in binding of WGA-FITC. Representative histograms from three independent experiments are depicted. (B) Summary of the results of three experiments showing changes in the median fluorescence (Med. fluor.) intensity of WGA-FITC binding at 0, 5, and 24 h at 22°C (dotted lines) and 37°C (solid lines). The differences at 5 and 24 h at 37°C were statistically significant. (C) Conidia were allowed to germinate in the presence or absence of chitinase for 24 h and then stained with WGA-FITC. **, P < 0.01.