FIG 2.

Characterization of murine immune responses against OPS2B1 and CPS2B1. Mice were immunized with various immunogens in Alhydrogel-CpG via the s.c. route on days 0, 21, and 35. Serum was obtained from mice 14 days after the final boost, and titers of IgG specific for B. pseudomallei LPS (A) or CPS (B) were determined by an ELISA. Individual symbols represent a single immunized mouse. The horizontal black lines represent the geometric means for each group (n = 15 for the OPS2B1 group, n = 14 for the CPS2B1 group, and n = 14 for unconjugated control groups). Significance at the 95% confidence level was determined by using a Mann-Whitney U test. LoD, limit of detection.