FIG 4.

Protective capacity of CPS2B1. Mice (n = 15 mice per group) were immunized with Alhydrogel-CpG alone (▲) or CPS2B1 formulated with Alhydrogel-CpG (△) via the s.c. route on days 0, 21, and 35. Five weeks after the final boost, mice were challenged i.p. with 1.06 × 10⁴ CFU of B. pseudomallei K96243. (A) Twenty-four hours after challenge, five mice from each group were culled, organs were removed, and bacterial burdens were determined. (B) The remaining 10 mice from each group were monitored until day 21 postchallenge, and survival was plotted. The horizontal black lines in panel A represent the geometric means for each group. Significance at the 95% confidence limit for organ bacterial counts was determined by using a Mann-Whitney U test, and that for survival was determined by using a log-rank (Mantel-Cox) test, as indicated (NS, not significant).