FIG 5.

Characterization of murine immune responses against CPS2B1 and LolC. Mice were immunized with various immunogens via the s.c. route on days 0, 21, and 35. Serum was obtained from mice 14 days after the final boost with a mixture of CPS2B1 and LolC formulated with Alhydrogel-CpG (▲), CPS2B1 formulated with Alhydrogel-CpG (△), or LolC formulated with Alhydrogel-CpG (◊), and titers of IgM and IgG specific for B. pseudomallei CPS (A) and LolC (B) were determined by an ELISA. Individual symbols represent a single immunized mouse (n = 10 for each group), except where no response was expected (anti-CPS in the LolC group and anti-LolC in the CPS2B1 group), where samples were pooled by cage (n = 2 for these groups). The horizontal black lines represent the geometric means for each group. Significance at the 95% confidence level was determined by using a Mann-Whitney U test and is indicated (NS, not significant).