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Immunoblot assays. Whole-cell lysates of wild-type M. catarrhalis and corresponding mutants are noted at the bottom. Blots were probed with rabbit antisera to recombinant SBP proteins (anti-SBP1, anti-SBP2, and anti-SBP3) as noted at the top of each panel. Molecular mass markers are noted in kDa on the left.
