FIG 7.

Results of flow cytometry with anti-SBP2 serum. The x axes show fluorescence intensity, and the y axes show cell counts. (A) Wild-type strain O35E (top panel) and sbp2 mutant (bottom panel) assayed with rabbit antiserum to recombinant purified SBP2 and preimmune serum. The mean ± SD of the percent change in geometric mean was 83.0% ± 16.9% for O35E, compared to −3.1% ± 5.1% for the sbp2 mutant (P < 0.01). (B) Representative data from flow cytometry among acute otitis media strains and their sbp2 mutants. Representative strains were strain 6349, which showed the highest percent change, and strain 5488, which showed the lowest percent change in whole-cell ELISA values of the 10 acute otitis media strains.