FIG 2.

The T2SS mutants exhibit protein secretion deficiencies, and 668 ΔgspD can be complemented in trans. (A) SDS-PAGE of filtered and concentrated LB broth supernatant proteins. Bacterial supernatant proteins were loaded on a 4 to 20% polyacrylamide gel, subjected to SDS-PAGE, and stained with colloidal Coomassie blue. The relative migrations of molecular mass protein standards (lane M; in kDa) are indicated on the left. (B) Detection of casein hydrolysis on 3% skim milk agar plate. The protein gel and the 3% skim milk plate are representative of at least 3 separate experiments.