FIG 4.

Immunoblot analysis of Hcp1 and TssM in B. pseudomallei supernatants. Strains were grown in LB for 8 h and supernatant proteins were separated by SDS-PAGE. The immunoblots were initially reacted with murine polyclonal anti-Hcp1 or murine polyclonal anti-TssM antisera and then with HRP-labeled goat anti-mouse IgG (γ). (A) Supernatant proteins from MSHR668, 668 ΔgspD, and 668 ΔgspE harboring pBHR2 (−) or pBHR2-virAG (+). (B) Supernatant proteins from MSHR668, 668 ΔgspD, and 668 ΔgspE harboring pBHR2-tssM. The images shown are representative of at least three separate immunoblotting experiments.