TABLE 2.
MSHR668 supernatant proteins absent, or appreciably reduced, in the MSHR668 ΔgspD supernatant following growth in rich (LB) and minimal (M9CG) broth
| Fold changea | Locus tag | Productb | Signal sequencec |
|---|---|---|---|
| Rich medium | |||
| 252 | BURPS668_A0845 | Peptidase M28E, aminopeptidase | Sec |
| 114 | BURPS668_2745 | Non-hemolytic phospholipase C (PlcN1) | Tat |
| 85 | BURPS668_A2859 | Peptidase S15, X-Pro dipeptidyl-peptidase | Tat |
| 65 | BURPS668_3144 | Peptidase S10, serine carboxypeptidase | Sec |
| 58 | BURPS668_A1560 | Bacterial extracellular solute-binding, family 1 | Sec |
| 49 | BURPS668_3129 | Putative uncharacterized protein | Sec |
| 40 | BURPS668_A2191 | Peptidase M4, thermolysin family | Sec |
| 37 | BURPS668_A0995 | Glycoside hydrolase, family 37 protein | NCS |
| 29 | BURPS668_A0507 | Purine nucleoside permease family protein | NCS |
| 27 | BURPS668_3846 | Putative lipoprotein, DUF3443 domain | Sec |
| 19 | BURPS668_3884 | Acid phosphatase (AcpA) | Tat |
| 18 | BURPS668_3613 | Peptidase M1, alanine aminopeptidase/leukotriene A4 hydrolase | Sec |
| 18 | BURPS668_1221 | Pectinacetylesterase family protein | NCS |
| 17 | BURPS668_3454 | Peptidase S10, serine carboxypeptidase | Sec |
| 17 | BURPS668_A0147 | PhoPQ-activated pathogenicity-related protein, PqaA type | NCS |
| 15 | BURPS668_A1338 | Six-hairpin glycosidase-like domain protein | NCS |
| 14 | BURPS668_0358 | Nonhemolytic phospholipase C (PlcN2) | Tat |
| 13 | BURPS668_2554 | Putative exported protein | Sec |
| 12 | BURPS668_A2736 | Neuraminidase/sialidase | Sec |
| 9 | BURPS668_1791 | Protease-associated domain, PA | Sec |
| 8 | BURPS668_A0822 | Levansucrase (SacB) | NCS |
| 8 | BURPS668_0540 | Putative β-N-acetylglucosaminidase; glycosyl hydrolase, family 20 | Sec |
| 8 | BURPS668_0751 | Cholesterol oxidase | Tat |
| 6 | BURPS668_1311 | Pectin lyase-like superfamily | Sec |
| 6 | BURPS668_A1638 | Peptidase S15, X-Pro dipeptidyl-peptidase | Sec |
| 6 | BURPS668_A2497 | Peptidase S8/S53 domain protein | NCS |
| 6 | BURPS668_A0869 | Peptidase M23A, B-lytic metalloendopeptidase | NCS |
| 5 | BURPS668_0744 | Ser/Thr protein phosphatase family protein | Tat |
| 5 | BURPS668_A1006 | Aldehyde oxidase/xanthine dehydrogenase family protein | Tat |
| 5 | BURPS668_A2658 | Bacterial Ig-like, group 1 domain protein | Sec |
| 4 | BURPS668_A2630 | Putative N-acetylmuramoyl-l-alanine amidase | Sec |
| 4 | BURPS668_2041 | Putative exported protein | Sec |
| 4 | BURPS668_A0106 | Putative uncharacterized protein | Sec |
| 3 | BURPS668_1946 | Exported chitinase; glycosyl hydrolase, family 18 | Sec |
| 3 | BURPS668_1435 | Putative nitrite/sulfite reductase | |
| 3 | BURPS668_2874 | Cysteine synthase K/M family protein | |
| 3 | BURPS668_3648 | Ubiquinol-cytochrome c reductase, cytochrome c1 | Sec |
| 3 | BURPS668_A2861 | Serine metalloprotease MprA (Peptidase S8, subtilisin-related) | Sec |
| 3 | BURPS668_A0846 | Metallopeptidase domain protein | Sec |
| Minimal medium | |||
| 20 | BURPS668_A0845 | Peptidase M28E, aminopeptidase | Sec |
| 16 | BURPS668_3129 | Putative uncharacterized protein | Sec |
| 7 | BURPS668_3846 | Putative lipoprotein, DUF3443 domain | Sec |
| 6 | BURPS668_A1560 | Bacterial extracellular solute-binding, family 1 | Sec |
| 6 | BURPS668_3918 | Cytochrome c5 | NCS |
| 5 | BURPS668_A0759 | Chitin binding domain protein | NCS |
| 4 | BURPS668_2270 | Putative lipoprotein, Ysc84 actin-binding domain | Sec |
| 3 | BURPS668_A2191 | Peptidase M4, thermolysin family | Sec |
| 3 | BURPS668_A1718 | Cytochrome cd1-nitrite reductase-like, haem d1 domain protein | NCS |
| 3 | BURPS668_A1062 | DJ-1/PfpI family protein | NCS |
| 3 | BURPS668_3884 | Acid phosphatase (AcpA) | Tat |
| 3 | BURPS668_1157 | GntR family transcriptional regulator/aminotransferase | Sec |
| 3 | BURPS668_1132 | Epoxide hydrolase-like family protein | |
| 3 | BURPS668_0776 | Glucose-methanol-choline oxidoreductase domain protein | |
| 3 | BURPS668_0766 | Glycosyl transferase, family 51 |
a
Values are MSHR668/668 ΔgspD ratios; a value of 1 was assigned to proteins that were not present or were present below the limit of detection, to prevent dividing by zero.
b
Putative functional analysis was predicted by InterPro (61), and products in bold were present in MSHR668 supernatants in both media.