FIG 4.

Cd14^−/− mice have an increased frequency of CD4⁺Foxp3⁺ T cells coincident with a decrease in the total CD4⁺ T-cell population. Splenocytes from naive (n = 5) or S. mansoni-infected (Sm) mice (12 weeks postinfection) (C57BL/6 and Cd14^−/−; n = 5) were surface stained using fluorescence-conjugated antibodies against CD4 followed by intracellular staining for Foxp3 or IL-10 protein, acquired by flow cytometry using a BD LSR II instrument, and analyzed using FlowJo (Treestar Inc., OR, USA). Frequencies of CD4⁺Foxp3⁺ of total CD4⁺ T cells (A), CD4⁺Foxp3⁺IL-10⁺ of total CD4⁺Foxp3⁺ T cells (B), and total CD4⁺ T-cell populations (C). Statistical significance was calculated using one-way ANOVA Tukey's multiple-comparison test. **, P < 0.01; ns, not significant. Results (means ± SEM) are representative of 3 or more independent experiments.