FIG 4.

Muc5B expression in the stomach of Helicobacter-infected and control mice. Expression of Muc5B in the antrum (A) and fundus (B) of the stomach of ASB1.4- and SS1-infected BALB/c mice is shown. Data are presented as fold changes in gene expression normalized to 3 reference genes and relative to the results for the negative-control group, which are set as 1. Data obtained from the time points 1 day, 4 days, and 1, 2, 3, 4, and 9 weeks are pooled and designated “early stage of infection.” Data obtained from the time points 12, 16, 20, and 24 weeks are pooled and designated “mid-stage of infection.” Data obtained from the time points 34 and 52 weeks are pooled and designated “late stage of infection.” Data are shown as means + standard deviations. Significant differences in expression levels between the infected groups and the negative-control group in a certain time frame (ANOVA) are indicated (*, P < 0.05; ***, P < 0.001). Significant differences in expression levels between groups inoculated with H. heilmannii ASB1 or H. pylori SS1 in different time frames (ANOVA) are indicated (#, P < 0.05; ###, P < 0.001).