TABLE 1.
Trial | Objective | Strain | Trial duration (wk) | No. of animals | Time point of sampling p.i.b | Data analyzedc |
---|---|---|---|---|---|---|
1 | Intestinal immune response | C57BL/6 | 3 | 15 infected mice and 5 negative controls | Wk 1, 2, and 3 | Cyst excretion and intestinal cytokine levels |
2 | Early intestinal immune response | C57BL/6 | 1 | 35 infected mice and 14 negative controls | Daily, starting day 1 p.i. until day 7 p.i. | Intestinal cytokine levels |
3 | Effect of IL17A on the intestinal immune response | C57BL/6 IL17RA-KO | 3 | 5 infected IL17RA KO mice and 5 infected controls | NA | Cyst excretion |
4 | Effect of PPARα deletion on the intestinal immune response | C57BL/6 PPARα KO | 3 | 5 infected PPARα KO mice and 5 infected controls | NA | Cyst excretion |
All trials or experiments were done with 6-week-old female mice.
Samples were taken from the small intestine for subsequent qRT-PCR analysis of the main cytokines. NA, not available.
Cyst counts of feces were performed daily for each animal.