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. 2014 Aug;88(16):9391–9405. doi: 10.1128/JVI.00934-14

FIG 2.

FIG 2

Latent HCMV alters monocyte cell lineage commitment. CD14+ monocytes that had been mock infected or TB40/E infected were harvested at 1, 3, and 6 days postinfection and subjected to flow cytometry analysis using fluorophore-conjugated antibodies to CD33 and CD14 (A) or CD163, CD169, and MHC class II polypeptides (B). Data compiled from three independent experiments are presented as the change (fold) in normalized mean fluorescence intensity (MFI) relative to that of day 1 mock-infected cells. All error bars show standard deviations (SD). (C) CD14+ monocytes infected with TB40/E or UV-irradiated TB40/E (TB40/EUV) were harvested 1 day postinfection and subjected to flow cytometry analysis using fluorophore-conjugated antibodies to CD14, CD163, and CD169. Gates represent the isotype control for each sample. (D) CD14+ monocytes that had been mock infected or TB40/E infected for 1 h at 37°C were placed into culture medium supplemented with 1,000 U/ml human IL-4 and 500 U/ml human GM-CSF for 6 days. Samples were subjected to flow cytometry analysis for CD14 and CD1a using the respective antibodies. Gates represent the isotype control for each sample.