FIG 1.

Molecular characterization of RhCMV 180.92 by variant-specific conventional and quantitative real-time PCR. Schematic representation of the UL/b′ region ORF and alignment of fibroblast-adapted RhCMV 180.92 (accession number DQ120516) to wild-type (WT) RhCMV (EF990255). Conventional PCR primer pair names (Table 1), along with positions of their respective amplicons, are shown above the UL/b′ region alignments. Control pGEM-T Easy plasmid for quantitative real-time PCR and location of the RhCMV 180.92 and B-region primer-probe sets are shown in the bottom. Forward and reverse primers for the RhCMV 180.92 amplicon are located within UL148 and rh167, respectively. Forward and reverse primers for the B-region amplicon are located within UL132 and the intron spanning UL132 and UL148, respectively.